Somaclonal variation, crop improvement, plant tissue culture. Tissue cultureassociated variation termed somaclonal variation has been observed in morphological and biochemical characteristics, and in chromosome number and structure 14, 21, 38, 46, 55. Early assessment of genetic fidelity in sugarcane saccharum. Dna methylation in sugarcane somaclonal variants assessed. Somaclonal variation studies in sugarcane somaclones by using. Somaclonal variation includes aneuploids, sterile plants and morphological variants, sometimes involving traits of economic importance in case of crop plants. Download as pptx, pdf, txt or read online from scribd. Muzaffar raza 1, rasheda aslam, ghazanfar hammad 1, ambreen ijaz3, m. Genetic variability is an important parameter for plant breeders in any con ventional crop improvement programme.
Detection of somaclonal variation in micropropagated plants of. Selected crops somaclonal variants, with increased disease resistance developed, without in vitro selection and within vitro selection are respectively given in tables 46. In this paper we describe this technology, the nature of the genetic variation thus far uncovered, and some current and future applications of somaclonal variation. Pdf somaclonal variation in sugarcane through tissue. Genetic variation found to occur between somaclones in plant tissue cultures was called somaclonal variation. The genetic variations found in the in vitro cultured cells are collectively referred to as somaclonal variations.
Somaclonal variation and crop improvement larkin and scowcroft 1981 proposed the term somaclone to describe the plants originating from any type of tissue culture. If youre looking for a free download links of somaclonal variation in crop improvement i. The study demonstrates that ssr genetic markers are the best tool for the investigation of genetic variation in sugarcane. Somaclonal variation for sugarcane improvement pooja manchanda, ajinder kaur, and satbir singh gosal abstract cell and tissue culture approaches serve as an important and an easily. In vitro generation of somaclonal variant plants of sugarcane. Feb, 2016 however, plant tissue culture may generate genetic variability, i. Applications of somaclonal variation nature biotechnology. Crop science abstract evaluation of in vitro propagated. Reducing somaclonal variation different steps can be used to reduce somaclonal variation. Somaclonal variations have largely contributed towards the development of disease resistance in many crops e. Download somaclonal variation in crop improvement i. In vitro generation of somaclonal variant plants of. In vitro studies on somaclonal variation and induced.
Somaclonal variations may reduce two years the time required for the release of new variety compared to mutation breeding. The occurrence of three types of somaclonal variants was observed. Agronomic evaluation of sugarcane lines transformed for. Co 94012 is a somaclonal variant of coc 671, with better sucrose content and mod. The aim of this study to evaluate genetic variation developed through soma clones and for this purpose three sugarcane. In vitro studies on somaclonal variation and induced mutagenesis. Somaclonal variation for sugarcane improvement springerlink. Tissue cultureinduced somaclonal variation of decreased. Somaclonal variation has been described for a variety of both qualitative and quantitative traits.
Genetic variations in plants that have been produced by plant tissue culture and can be detected as genetic or phenotypic traits. Box 18, chiuju, pingtung taiwan abstract an improvement program for disease resistance and horticultural traits in cavendish banana cultivars was established using somaclonal variants as a source of variation. A selected list of disease resistant crop plants obtained from somaclonal variations without in vitro selection along with the pathogenic organisms is given in table 46. The study was carried out to investigate the response of different sugarcane. Sugarcane is commercially important and major cash crop of pakistan. Somaclonal variation and induced mutations in crop. Read this article to learn about the basis, isolation and limitations of somaclonal variations and also factors affecting the production of somaclonal variants. Pdf somaclonal variation in sugarcane through tissue culture and. Jun 23, 2018 this variation arising from cell and tissue cultures is called somaclonal variation that can be geneticepigenetic in nature.
The use of only one type of molecular marker, such as rapds, to assess the genetic stability of an in vitro production system may be inadequate, and an approach that focuses on morphological traits appears to be a valuable. A total of four plants regenerated from the tolerant calluses were selected but the best of them in vigor grown in in vitro and hydroponic systems under. Somaclonal variation is the variation seen in plants that have been produced by plant tissue culture. Changes of character that occur due to somaclonal variation and mutation happen in random. Callogenesis is a helping tool in tissue culture for creating variation. Saurabh bhatia, kiran sharma, in modern applications of plant biotechnology in pharmaceutical sciences, 2015. Please view the pdf by using the full text pdf link under view to the left. Somaclonal variations and their applications in horticultural. It is well known that increasing numbers of subculture increases the likelihood of somaclonal variation, so the number of subcultures in micropropagation protocols. Somaclonal variation can be manifested as either somatically or meiotically stable events. Eight genotypes of saccharum officinarum were crossed with saccharum spontaneum and 14 genotypes of s. A case study with olive 125 whereas in vitro the loss of cellular control, which gives rise to disorganised growth, is a characteristic of somaclonal variation 6,19. Somaclonal variation and in vitro mutation induction. Somaclonal variation as a tool for crop improvement springerlink.
Advantages of somaclonal variations help in crop improvement creation of additional genetic varitions increased and improved production of secondary metabolites selection of plants resistant to various toxins, herbicides, high salt concentration and mineral toxicity, and disease resistance. Somaclonal variation in sugarcane through tissue culture and evaluation for quantitative and quality traits article pdf available in euphytica 1681. Variations in number and structure of chromosomes are commonly observed. Variation in sugarcane plant derived from cell cultures was reported as early as 1969 by researchers. Proc int soc sugar cane t echnol 157 kumar p agarwal a t iwari ak et al 2012 possibilities of development of red rot resistance in sugarcane through somaclonal variation. Somaclonal variation and in vitro mutation induction have been used in sugarcane, banana and potato, with the objective of improving specific characters, mainly the resistance to diseases and other defects that limit the use of some important commercial varieties. Plant tissue culture or micropropagation technique is the rapid method to multiply newly released cultivar in limited time. Somaclones of nia1198 were developed through callus culture and subjected to field trials under randomized complete block design with three replications over two consecutive years 2014 and 201415. Somaclonal variation caused by the process of tissue culture is also called tissue culture induced variation to more speci. Similarly, genetically transformed cells are carefully screened to avoid unwanted and unintended somaclonal variations so that the commercially released clones have only beneficial effects. Sugarcane, somaclonal variations, red rot, scmv, candidate genes, genetic integrity. Ahmed hk 2004 somaclonal variation in sugarcane through tissue culture.
Somaclonal variation in micropropagated heliconia bihai cv. Release of plant germplasm generated through somaclonal variation. Crop science abstract evaluation of in vitro propagated sugarcane hybrids for somaclonal variation 1 view my binders. Their variability was manifested according to the data for stalk diameter, length, internodes, millable canes, leaf length, width and sucrose characters. Somaclonal variation in tissue culture is a complex problem that needs several approaches to be appreciated correctly. Agronomic evaluation of sugarcane lines transformed for resistance to sugarcane mosaic virus strain e r. However, plant breeders have successfully recombined the desired genes from. Crop improvement by conventional method in vegetatively propagated crops like sugarcane is very difficult due to its narrow.
Presentday commercial cultivars of sugarcane saccharum spp 2n 99. Although the direct formation of plant structures from meristem cultures, without any. D student, agronomy and plant breeding department, college of agriculture, shahid chamran university, ahvaz, iran. The term somaclonal variation is a phenomenon of broad taxonomic occurrence, reported for species of different ploidy levels, and for outcrossing and inbreeding, vegetatively. Pdf assessment of genetic variability in somaclonal. It is well known that increasing numbers of subculture increases the likelihood of somaclonal variation, so the number of subcultures in micropropagation protocols should be kept to a minimum. Epigenetic nonheritable variations variations generated during tissue culture caused by temporary phenotypic changes occur at low frequency 4. Somaclonal variation can provide an alternative for improvement of existing genotypes shahid et al. Somaclonal variants of several crops have been successfully obtained by this approach e. The field grown somaclones were characterized for yield and quality. No somaclonal variation could be detected among plantlets submitted to virus elimination programs. Tissue culturederived variation in crop improvement pdf. Type and concentration of applied plant growth regulators pgr optimal concentration and precise ratios of auxins and cytokinins is essential for efficient micropropagation skoog and miller 1957. Crop science abstract evaluation of in vitro propagated sugarcane hybrids for somaclonal variation 1 view my binders this article in cs.
Pdf somaclonal variation as a tool for crop improvement. As a result of somaclonal variations, several novel variants of existing crops have been developed, e. Somaclonal variations was a good source of genetic improvement of sugarcane for disease resistance with no snp changes in candidate genes. Somaclonal variation for sugarcane improvement read online at passwordsafe. Callus regeneration, vegetative transmission, sugarcane. Isd16 mohashweta roy1, monzur hossain2, animesh biswas1, rafiul islam2, shipra rani sarker2 and sharoni akhter3 1department of botany, govt. Jan 28, 2009 eight genotypes of saccharum officinarum were crossed with saccharum spontaneum and 14 genotypes of s. Transfers were made at 30day intervals for 1, 2 or 3 subcultures. Chromosome number somaclonal variation sugarcane variety intergeneric hybrid sugarcane cultivar. Somaclonal variation for disease resistance in wheat and. Click download or read online button to get somaclonal variation in crop improvement ii book now. Applications of somaclonal variations biology discussion. Article induction and evaluation of somaclonal variation. The usefulness of variation was first demonstrated through the recovery of disease resistant plants in potato resistance against late blight and early blight and sugarcane resistance against eyespot disease, fiji disease and downy.
The somaclonal variation rate forheliconia bihai cv lobster claw i, under the proposed conditions, was 61. Somaclonal variation for sugarcane improvement ebook pdf. Sources of variations detected in plant tissue culture. Identification of somaclonal variants of sugarcane. For the detection of somaclonal variation, 38 primers pair were used and 15 simple sequence repeats ssr primer pairs were found to be polymorphic with 51. Somaclonal variants resistant to sugarcane mosaic virus scmv were obtained from susceptible sugarcane cv pr62258 through somatic embryogenesis by increasing the number of subcultures of the embryogenic callus tissue in ms medium with 3 mgl 2,4dichlorophenoxyacetic acid. Somaclonal variation in crop improvement ii download. Detection of somaclonal variation in micropropagated.
Co 94012 a new sugarcane variety through somaclonal variation. Extensive examples are discussed of such variation in culture subclones and in regenerated plants somaclones. The important applications of somaclonal variations are briefly described. The occurrence of somaclonal variation is both a boon and bane. Somaclonal variation a novel source of variability from. Tissue culture technique was used to obtain salt tolerant variants from embryogenic calluses of sugarcane saccharum sp. This site is like a library, use search box in the widget to get ebook that you want. Transformation of foreign gene in sugarcane variety isd 28 using agrobacterummediated method. Detection of somaclonal variation in micropropagated plants of sugarcane and scmv screening through elisa abdullah 1, smiullah, farooq ahmad khan1, rameez iftikhar2, m. Angra 2 1 instituto agronomico do paranaiapar, caixa postal 481, 8600970 londrina, pr, brasil. Somaclonal variation is a tool that can be used by plant breeders.
Nevertheless, progress in the adoption of such technology in the generation of mutant accessions has been extremely limited. Tissue culture is an efficient method of clonal propagation. Somaclonal variation a novel source of variability from cell cultures for plant improvement. These somaclonal variations are mainly caused by newly generated mutations arising from tissue culture process sato et al. According to the increasing demand of sugarcane somaclonal variation play vital role to overcome problems for sugarcane breeding. Sep 23, 20 tissue culture is a powerful tool in plant gene transformation and molecular breeding. These 39 hybrids were raised in the field and used as donor clones for in vitro culture studies. In vitro induction and characterization of somaclonal. Application of somaclonal variation in crop improvement programme was established in sugarcane. The term somaclonal variation is a phenomenon of broad taxonomic occurrence, reported for species of different ploidy levels, and for outcrossing and inbreeding, vegetatively and seed propagated, and cultivated and non. Somaclonal variation free download as powerpoint presentation. Abouzid abstract zki 1993 noted that genetic engineering in sugarcane.
One could expect to find a certain degree of variation, since almond plantlets were. In the present investigation, optimal media compositions for enhancing the callus induction and subsequent plant regeneration were worked out in sugarcane saccharum spp. The occurrence of subtle somaclonal variation is a drawback for both in vitro cloning as well as germplasm preservation. Callus tissueorganogenesisregenerated plants hardening and selfingsomaclonal variantssteps involved in induction and selection of somaclonal variations. In vitro regeneration, detection of somaclonal variation and screening for mosaic virus in sugarcane saccharum spp. Somaclonal variation is an important tool for creating genetic diversity in sugarcane. Advantages somaclonal variations are stable and occur at high frequencies. Somaclonal variation in sugarcane through tissue culture and evaluation for. Article induction and evaluation of somaclonal variation in sugarcane saccharum officinarum l. Somaclonal variation appears to be an important alternative for creation of genetic variability in crops where tissue culture plant regeneration system has been established. Chromosomal rearrangements are an important source of this variation. Variation of the chlorophyll in the leaf, low stature variant and pseudostem and petiole color variant, the latter with ornamental potential.
Somaclonal variation for disease resistance in wheat and production of dihaploids through wheat x maize hybrids. Sugarcane improvement through induced mutations using vegetative propagules and cell culture techniques. Somaclonal variation an overview sciencedirect topics. These clones have different discard percentages and were categorized according to the in vitro visual frequency of somaclonal variation symptoms abnormal in vitro plantlets in two groups. Read somaclonal variation as a source of resistance to eyespot disease of sugarcane, plant breeding on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Cp48103 that cultured on a selective medium containing different levels of nacl 0, 0. In vitro regeneration, detection of somaclonal variation and. It is argued that this variation already is proving to be of. Because there is a chance of result of unwanted offtypes that reduces the commercial value. The grown up plants were transplanted to well prepared. Phenotypic and genotypic variations, collectively called somaclonal variations, have long been known to be induced during dedifferentiation and regeneration of plants in tissue cultures larkin and scowcroft 1981. In vitro generation of somaclonal variant plants of sugarcane saccharum spp. Variation in chromosome numbers has been frequently observed in longterm plant cell cultures.
Somaclonal variation, therefore, can arise from somatic mutations already present in the donor plant karp 1994. The hawaian sugar planters association experimental station recorded genetic variation among sugarcane plants regenerated from tissue culture. Evaluation of somaclonal variation in almond using rapd and. Somaclonal variation is an intrinsic phenomenon that must be taken into consideration in any program for sugarcane improvement through genetic engineering. Variations were recorded based on cytogenetic, morphological and enzyme profiles. Assessment of genetic variability in somaclonal population of sugarcane. Pdf in vitro regeneration, detection of somaclonal. Induced mutations and somaclonal variation in sugarcane. Increasing altolerance of sugarcane using ethyl methane. Somaclonal variation is considered as a complex phenomenon resulting from various genetic and cellular mechanisms under in vitro conditions. Somaclonal variants of sugarcane are available for several traits like drought, salt. During plant propagation process under laboratory conditions, the genomic shock is induced which can result in the activation of various transposons, retrotransposons, chromosomal changes, methylation and demethylation of dna leading.